Primordial odontogenic tumor with prominent calcifications.

Primordial odontogenic tumor (POT) is a rare mixed odontogenic tumor composed of primitive ectomesenchyme similar to the dental papilla. The outer surface consists of columnar/cuboidal odontogenic epithelium similar to the inner enamel epithelium, and there is no hard tissue formation. Until now, 27 cases have been reported in the English literature. This article describes the clinicopathological characteristics of one case of POT, representing the oldest patient (aged 26 years) reported to date.

[Soluble programmed death-1 and soluble programmed death ligand 1 protein expression and immune status in patients with recurrent aphthous ulcer].

OBJECTIVE: This study aims to investigate the possible role and significance of soluble programmed death-1 (sPD-1)  /soluble programmed death ligand 1 (sPD-L1) in the immune pathogeneses of recurrent aphthous ulcer (RAU). METHODS: A total of 30 RAU cases (18 cases of minor RAU, 5 cases of major RAU, and 7 cases of herpetiform ulcers) were enrolled in this study. A total of 18 healthy people served as controls. Lymphocyte subsets (CD3⁺, CD4⁺, CD8⁺, CD19⁺, and CD16⁺+56⁺) were investigated by flow cytometric analysis. Humoral immunity (IgG, IgA, IgM, C3, and C4) was explored by nephelometry immunoassay. The sPD-1 and sPD-L1 protein levels in the sera of RAU patients were investigated by enzyme-linked immunosorbent assay. The correlations of the sPD-1 and sPD-L1 protein levels with the immune status and clinical characteristics of the RAU patients were analyzed by SPSS 19.0. RESULTS: The number of CD4+ T cells decreased and the levels of IgM antibodies increased in the RAU patients relative to those in the normal controls (P<0.05). The sPD-1 and sPD-L1 protein levels in the RAU patients were significantly higher than those in the control group (P<0.05). Meanwhile, the sPD-1 and sPD-L1 protein levels in the patients with minor and major RAU were significantly higher than those in the control group (P<0.05). By contrast, no significant difference was found in the patients with herpetiform RAU (P>0.05). Positive correlations were noted between the sPD-1 protein level and the CD19+ cell frequency or C4 level (r1=0.389, P1=0.034; r2=0.382, P2=0.037). CONCLUSIONS: Cellular immune hypofunction and humoral immunity disorders were found in the RAU patients. The PD-1/PD-L1 signaling pathway, which might be influenced by the involvement of sPD-1 and sPD-L1 proteins to a certain extent, may play some roles in the immune pathogenesis of RAU.

Preoperative diagnostic value of magnetic resonance imaging for fungal ball of nasal sinuses / 局解手术学杂志

Objective To investigate the magnetic resonance imaging (MRI)features and pathologic characteristics of the fungal ball (FB)of the nasal sinuses,for the purpose to provide reference for surgical treatment.Methods The MRI data of 27 patients of nasal sinuses who were diagnosed by surgical pathology were retrospectively analyzed.Of 27 patients,there were 14 male and 13 female,aged from 49 to 86,anverage age 57.6,with course of disease from 6 months to 5 years.All patients preoperatively recieved 1.5 T high field strength mri scan and enhanced scan and functional nasal endoscopic surgical radical resection lesions.Results Maxillary sinus disease,which unilateral 92%(25 /27),bilateral 4% (1 /27),sphenoid sinus disease 4%(1 /27),FB was located in the sinus cavity central.FB isointense signal (com-pared with the white matter of the brain),hypointense on T2WI,DWI (b value =1000)was mixed high and low signal,Gd DTPA enhanced no enhancement,with around mucosal thickening isointense signal,high signal intensity on T2WI,ring enhancement.The histopathology slices of 27 cases were found wrapped fungal hyphae,which confirmed for FB.The pathological basis of FB was fungal silk containing manganese (paramagnetic substances),FB calcium phosphate and calcium sulfate and other substances,due to the magnetic susceptibility effect of T2WI was low signal,DWI high and low mixed signal changes.Conclusion MRI can reflect the histopathological characteristics of FB,and it is of great value in diagnosis and differential diagnosis,as well as providing important imaging evidence for patients undergoing surgical treatment.

Selection and Identification of the Biological Characteristics of a Cold-adapted Genotype G1P8 ZTR-68 Rotavirus by Serial Cold-adapted Passaging / 病毒学报

We wished to select a cold-adapted genotype G1P[8] ZTR-68 rotavirus (China southwest strain) in MA104 cells for possible use as a live vaccine. ZTR-68 was recovered originally from children with diarrhea. The virus was cultivated at 37 degrees C at the first passage. Then, the cultivation temperature was decreased stepwise by 3 degrees C per eight passages. In total, the virus was passaged 32 times, and cultivation was terminated at 28 degrees C. Biological characteristics of the virus were analyzed during serial passages. There was no difference between the migration patterns of genomic dsRNA segments according to polyacrylamide gel electrophoresis of original and cold-adapted viruses. Infectious and red cell-agglutination titers of cold-adapted virus were lower than those of the parent virus. Also, the virus formed small-size plaques with irregular shapes at 31 degrees C and 28 degrees C. These results suggested that a genetically stable attenuated virus can be obtained through serial cold-adapted passages. Thus, an alternative strategy is provided by cold-adaption for development of attenuated live rotavirus vaccines.

Feasibility of intravenous administration of aspirin in acute coronary syndrome / 老年心脏病学杂志（英文版）

s To compare the clinical effects of intravenously and orally administered aspirin in the treatment for acute coronary syndrome (ACS),and to evaluate the adverse effects of intravenous administration of aspirin.Methods One hundred and twenty-five patients with unstable angina pectoris or acute myocardial infarction were randomized into three groups:group 1 received intravenous aspirin (300mg/d,n =40),while groups 2 (n =42) and 3 (n =43) received orally administered aspirin (100mg/d and 300mg/ d,respectively).The control group included 30 patients with no heart disease or blood disease,and they had never taken aspirin and clopidogrel.Blood samples were taken at 2nd and 7th day of hospitalization.Platelet aggregation and the level ofplatelet activation marker CD62p were measured and compared among the groups.Patients were followed up for 6 months for the occurrence of major adverse cardiovascular events.Results There were no statistically significant differences in the decrease in adenosine diphosphate (ADP)-induced platelet aggregation rate (12.01±10.45%,6.76±14.62% and 9.73±16.72% for group 1,group2 and group 3,respectively),the decrease in arachidonic acid (AA)-induced platelet aggregation rate (6.73±11.34%,6.95±12.45% and 7.57±13.11%,respectively),and the decrease in CD62p level (10.89±18.62%,8.92±11.57% and 7.05±15.67%,respectively).At six months,there were 4 deaths (10%) in group 1,4 deaths (9.5%) in group 2 and 5 deaths (11.6%) in group 3 (P＞0.05).Conclusions Intravenous administration of aspirin provides a new approach as an anti-platelet treatment for ACS patients,especially those who can not tolerate oral administration of aspirin.(J Geriatr Cardiol 2008;5:212-216)

Nursing Management in Nosocomial Infection Control / 中华医院感染学杂志

OBJECTIVE To study effect of nursing management's function in nosocomial infection control and supervision.METHODS Nursing managers made good use of their management function to control hospital infection by staff training,controlling the quality of nursing work,and working out nursing skill standards.RESULTS Nursing management increased the consciousness of hospital infection control among nursing staff and decreased the rate of nosocomial infection effectively.CONCLUSIONS The quality of nursing works affects the efficiency of the control of nosocomial infection.During the process of nosocomial infection control,nursing department plays an important role and can't be replaced.

Evaluation of Diagnostic Value of CT in Osteoarticular Tuberculosis / 实用放射学杂志

Objective To evaluate CT diagnostic value of osteoarticular tuberculosis. Methods The clinic informations,X-ray findings and CT features in 112 cases of osteoarticular tuberculosis were retrospectively analyzed. Results The lesions localized at spine in 90,hip joint in 7,sacroiliac articulation in 6,knee joint in 5,wrist joint in 3 cases and ankle joint in 1.X-ray findings included:widening,narrowing or disappearance of joint space,swelling of soft tissue,osteoporosis,bone destruction with sclerosis of edge,sequestrum,forming of cold Abscess and calcification in Abscess.CT manifestations :synovial articular tuberculosis manifested thickening articular capsule,hydrarthrosis and swelling of soft tissue ;and bony articular tuberculosis basically manifested as the same as X-ray finding.Conclusion CT can demonstrate the features of osteoarticular tuberculosis,like sequestrum and cold Abscess, as well as the degree and extent of bony destruction,which play an important role in diagnosis of osteoarticular tuberculosis,especially in the early stage .

CT Features of Body Echinococcosis at Plateau of Qinghai / 实用放射学杂志

Objective To analyse the CT features of body echinococcosis at plateau of Qinghai.Methods CT findings in 108 patients with echinococcosis were retrospectively analyzed.Results In 108 cases,there were 101 cases of cystic echinococcosis and 7 cases of alveolar echinococcosis which manifested as irregular mixed-density masses.The 101 cases of cystic echinococcosis were divided into five types:26 cases of unilocular or simple cystyc type,15 cases of multilocular or separate inner cyst,37 cases of sub-polycystic,19 cases of calcification of parenchyma and 11 cases of mixtures.The most of them in 108 cases were liver echinococcosis,others included multi-organs of abdomen and pelvis in 18,lung in 7,lung and pericardium in one and tibia echinococcosis complicated with infection.The data in this group showed that echinococcosis in abdomen and pelvis was higher than that in lung.Conclusion CT can definitely demonstrate the site and nature of body echinococcosis and the diagnosis was not difficult.

IDENTIFICATION OF STEM CELL ISLANDS IN REGENERATED EPIDERMIS / 解放军医学杂志

To identify the stem cell islands in regenerated epidermis, the same biopsies used in our previous experiments were used in this study. CD87 immunohistochemicy and PAS staining were used. The scant CD87 positive cells could be found in basal membrane. Also, the positive staining of PAS could be seen in the basal membrane in both normal and regenerated epidermis. No CD87 positive cells were found in the spinous and granular layers. The results indicate that the error in metrology could be excluded from both CD87 immunohistochemicy and PAS staining, and that the stem cell islands may come from the cell reversion in regenerated epidermis.